Reporter
Part:BBa_K2269005
Designed by: José Álvaro Ballesteros González Group: iGEM17_Valencia_UPV (2017-09-10)
Assembly Reporter Last Part 3/3 (Ros1)
Unit transcription for the constitutive expression of a chimeric construct comprising a DNA binding protein fused to A. thaliana PIF6 (amino acids 1-100) and a nuclear localization sequence.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 276
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 276
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 276
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 276
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 276
Illegal AgeI site found at 132 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |